![]() Most protein separations are performed using a 'discontinuous' (or DISC) buffer system that significantly enhances the sharpness of the bands within the gel. ![]() Because of practical limitations, protein electrophoresis is generally not suited as a preparative method. Gel electrophoresis is often performed in combination with electroblottingimmunoblotting to give additional information about a specific protein. Each method has many variations with individual advantages and limitations. ![]() The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis (in short: gel electrophoresis, PAGE, or SDS-electrophoresis), free-flow electrophoresis, electrofocusing, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Protein electrophoresis is a method for analysing the proteins in a fluid or an extract.
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